Stem Cells And Regenerative Medicine Cord Blood Banking...

Name: Darshana Chaudhary Student Number: s5024975 Course: 7001HSV Applied Research Methods Tutor: Jennifer Boddy Due Date: 14th September 2015 Date Submitted: 14th September 2015 Word Count: 1671 words STEM CELLS AND REGENERATIVE MEDICINE : CORD BLOOD BANKING AND ITS THERAPEUTIC APPLICATION Abstract : Stem cells can give rise to any tissue found in the body and thus provide nearly limitless potential for medical applications . Stem cells have the great potential to develop into many different cell types in the body during early life and growth. They serve as a repair system . Due to unlimited therapeutic applications of stem cells , they continue to be of great importance scientifically and†¦show more content†¦Human embryonic stem cells . Patient-specific therapy. METHOD : Articles for this review were selected from Pubmed Central and Medline databases and proquest databases. Introduction : Stem cells are able to generate mature terminally differentiated cells of atleast one lineage and possess the capacity of self-renewal .Stem cells can be categorized as totipotent, pluripotent or multipotent according to their developmental potential. (Takahashi and Yamanaka 2007). Stem Cell Classification Based on Differentiation Potential: Stem cells can be classified based on their ability to differentiate. They are classified into following five types : Totipotent Cells: Totipotent cells are found in early stage of development .The totipotent cells forms the embryo and the placenta and differentiate into both embryonic and extraembryonic tissues. Pluripotent Cells: Pluripotent cells differentiate into cells that arise from three germ layers called – ectoderm , endoderm and mesoderm , from which other tissues and organs develop. Oligopotent Cells: oligopotent are capable of self renewal and differente into many tissue types. Unipotent Cells: unipotent cells are capable of self renewal and differentiate into only one single type of cell. Multipotent Cells: They are found in most tissues and differentiate into cells from a single germ layer. (Kolios, 2012) Sources of Stem Cells for Regenerative Medicine: There are a number of cardiovascular , neurological

The US World War The Usas Entry To WW1 - 945 Words

Although Russia’s withdrawal proved a significant blow, the USA’s entry to WW1 on the 6th April 1917 contributed not only a mass of fresh troops and artillery but a monumental rise in previously fading allied morale. Following the outbreak of WW1 in July 1914 Woodrow Wilson, US President, announced the nations neutrality from involvement in European conflict, but in January 1915 Germany declared its policy of unrestricted submarine warfare to counter the British naval blockade. On the 7th May, the Lusitania, a British liner carrying approximately 173 tons of British war munitions was torpedoed by a German U-Boat killing 1201 civilians, 128 of which were American. The following day the New York Times wrote â€Å"In the history of war, no†¦show more content†¦After the dawn of the Russian Revolution in March 1917 the countries politics entered a state of extreme turmoil. FAfter failing to seize power from the provisional government in the July Days uprising Vladimir Lenin’s Bolshevik Party finally attained seized power in the 1917 October Revolution creating a communist government.. In order to retain leadership Lenin planned to implement his April theses which contained 3 fundamen tal promises to the Russian people; peace, land and bread and on the 3 March 1918 the . In the Treaty of Brest-Litovsk was signed, formally ending Russian involvement in the war. The terms were initially deemed too harsh and fighting resumed momentarily along the Eastern Front until Lenin realised Russia, in its weakened state, would be forced to submit to enemy conditions. Such terms included the loss of approximately one million square miles of Russian territory, a third of Russias population, the majority of its coal, oil and iron and much of its industries. Lenin bitterly coined the settlement â€Å"that abyss of defeat, dismemberment, enslavement and humiliation.† With Russia now withdrawn from the war, the central powers were provided with a ‘window of opportunity’ as they no longer faced a ‘war on two fronts’. This allowed them to relocate a mass of troops to the Western Front to support a mass offensive in hopes they could end the war before the US arrived in sufficient strength to ensure allied victory.Show MoreRelatedThe Great War Of The United States1883 Words   |  8 PagesFirst World War, the USA was a flourishing power in terms of having one of the largest economies and military strength, however America had few foreign policy ambitions during this period. Despite purchasing Alaska from Russia in 1867, there was no real interest in expanding America. Before The Great War, it seemed unlikely that America would become involved in war over the conflicts of other western powers. They considered themselves a ‘City on the Hill’, an example to the rest of the world of stabilityRead MoreCauses of the U SA Boom in the 1920s Essay1089 Words   |  5 PagesAmerica was WW1. The allies just didn’t have the resources to produce the weapons needed to continue the war effort themselves. Therefore they bought the required munitions etc. off the Americans, so as a result the Americans where being pumped full of money from the allies. This meant that while the allies fought the war and were being depleted of their wealth the Americans had a time of prosperity resulting in the USA finishing WW1 as the richest country in the world, while

Leonardo da Vinci in our life Essay Thesis Example For Students

Leonardo da Vinci in our life Essay Thesis Leonardo dad Vines contribution to the era of Renaissance The Renaissance era created an immense amount of talented scientists, artists, inventors, and philosophers who contributed great deal to the development of human race. In history it is hard to find a similar genius individual as the creator of High Renaissance art Leonardo dad Vinci. Even afterwards of research on his creations, we are still captivated by the brilliance of his works. Leonardo dad Vinci was an artist, a sculptor, an architect, a philosopher, a historian, a mathematician, a physicist, a mechanic, and an astronomer. Consider Leonardo a genius of the High Renaissance. He was undeniably way ahead of his time, and is undoubtedly one of desegregates geniuses that ever lived. He played an enormous role in the development of art and science of the High Renaissance in Italy. His creations Last Supper (495-97) and Mona Lisa (1503-06) are among the most widely popular art works of the Renaissance. The Mona Lisa painting is a good example of his work which contributed to the development of art in the Renaissance. The Mona Lisa is a portrait of a wife of a Florentine noble. She refused to smile; Leonardo even tried ring musicians but that didnt change her mind. At last, Just for a second she smiled faintly and Leonardo was able to capture it. In his drawing Leonardo masters the techniques of Suffuse and Chiaroscuro. Suffuse involves the most gradual transition from one color to another, giving it a very delicate and expressive image. As mentioned in the studies, Chiaroscuro highlights the contrast of light and shadow. In the Mona Lisa, this is most evident in the contrast between the face and the dark background. I consider the Mona Lisa his most beautiful creation. Leonardo dad Vinci highly influenced the development of science of the High Renaissance in Italy. He is considered one of the prominent contributors to the scientific discoveries of the era. Dad Vinci extended his studies into science and other subjects. For example, he drew complex models of flying machines, as he was fascinated by flight. He also attempted to build flying objects himself and designed an immense amount of mechanical devices. Based on his studies of birds, he drew a human powered ornithology, which is a wing flapping machinate would fly, mimicking the movement of a bird in flight. As a result, some of the machines that he drew on paper such as helicopters would become reality many centuries later. He also had knowledge in the studies of anatomy and was one of the first to understand the circulation of blood in the human body. There seemed to be no limit to his interests and work. Leonardo dad Vinci is one the most prominent individualists lived in the Renaissance era and contributed a vast amount of scientific discoveries and beautiful works of art which are genius and timeless. Leonardo looked far ahead of his time and influenced our generation and development of the modern world today. Leonardo dad Vinci in our life By Andrews

Pollution Essays (457 words) - Nuclear Power, Nuclear Technology

Pollution Pollution. What is pollution? According to the Merriam Webster Dictionary pollution is ?the action of polluting esp. by environmental contamination with man-made waste.? The United States runs on power. Much of the United States' powers come from various nuclear power plants all across the country. Some power plants are getting old and are not up to date. These power plants are emitting various gasses and chemicals into the air. Transcendentalists believe that people should coexist with nature. They believe that nature is an entity of equal importance to us, and not just our supply of resources. The problem is that the gasses and other pollutants released are harming our environment. There are many solutions to this problem. Some solutions that might have been made by the transcendentalists would be to shut down nuclear power plants completely. Another solution would be to update the current problems or find better power sources. The first solution a transcendentalist would have would be to totally abolish the use of nuclear power plants. This idea is pretty basic in its nature. If all the nuclear power plants in America were shut down, there would be less cases of pollution. Though it may not seem it, the best way to deal with pollution, is with ?simplicity, simplicity, simplicity,? as Thoreau says in ?Walden.? By merely closing all the nuclear power plants in the United States there is no chance for pollution to spread. This idea is the perfect transcendental idea. If people went away from technology and escaped back to reality they would find that problems could be solved very easily. Many transcendentalists believed in ?simplicity? and the carefree life. Some transcendentalists believed in nature as our future and our offspring. Another possible transcendental solution would be to update current situation. Some transcendentalists would believe in modernizing and renovating the existing power plants so that they would be more efficient and nature friendly. Nature needs to be taken care of and so instead of totally disposing of nuclear power plants, they could be made to friendly to Mother Earth. As Emerson says in ?The Over-Soul?, ?the only prophet of that which must be, is that great nature Nature is essential to the survival of the human race. Why should society kill something that it requires? Humankind ought to learn that by killing nature it is only killing itself. Nuclear power plants should be renewed rather than destructed. Briefly, the transcendentalists would have had many solutions for the problem of nuclear pollution to the environment. Two of these solutions would have been to close the power plants or revise the current power plants to make them more energy and nature conservative. These two solutions would work best with the problem. English Essays

Frederick McKinley Jones

Frederick McKinley Jones Frederick McKinley Jones was one of the most prolific black inventors and held over 60 patents at the time of his death. Some of his most important work changed the way we store and transport our food, and altered the transportation and grocery industries forever. Fast Facts: Frederick McKinley Jones Born: May 17, 1893 in Cincinnati, OhioDied: February 21, 1961 in Minneapolis, MinnesotaKnown For: Inventor who revolutionized the refrigeration industry and held over 60 patentsEducation: Orphaned at a young age, Jones had little formal education, but he taught himself automobile mechanics and became an engineerAwards and Honors: First African American elected to the  American  Society  of Refrigeration Engineers, and first African American to be awarded a National  Medal  of Technology (posthumously)   Early Years A native of Cincinnati, Ohio, Frederick McKinley Jones was born on May 17, 1893, to an Irish father, John Jones, and an African American mother. By the time he was 7 years old, his mother had deserted the family, and his father sent him to live with a Catholic priest in a rectory in Covington, Kentucky, just across the Ohio River from Cincinnati. While in Kentucky, young Fredericks father passed away, essentially leaving him an orphan. When he was 11, Jones decided hed had enough of living with the priest, so he ran away and returned to Cincinnati. During his teen years, he found work doing odd jobs around the city, and soon found he had a natural aptitude for automobile mechanics. He also began to read a lot, although he had little formal education. At 19, he traveled north to a farm in Hallock, Minnesota, where he took a job doing mechanical labor on the farm machinery, and soon was able to obtain an engineering license. When war broke out, Jones enlisted in the U.S. Army, where he was in high demand for his mechanical abilities. He spent much of the war making repairs to machines and other equipment, as well as maintaining communications systems at the front. After his military service ended, he returned to the farm in Minnesota. Inventions While living at the Hallock farm, Jones began to take an interest in electronics, and read as much as he could on the subject. According to Biography.com, When the town decided to fund a new radio station, Jones built the transmitter needed to broadcast its programming. He also developed a device to combine  moving  pictures with sound. Local businessman Joseph A. Numero subsequently hired Jones to improve the sound equipment he produced for the film industry. Numeros company, Cinema Supplies, was excited about Jones inventions, and within a few years, the two of them would form a partnership. Frederick McKinley Jones, inventor, refrigeration expert and cofounder of Thermo King Corp., holding a model of his refrigerated railroad car. Bettmann Archive / Getty Images Mobile Refrigeration In the 1930s, it was risky to transport perishable products. Grocery shipments were typically limited to short distances; ice melted quickly, and any sort of  electronic refrigeration unit required a layover at a power source, which delayed delivery time. However, by 1938, Jones believed he had found a solution, and in 1940 he obtained a patent for the first practical transport refrigeration unit for the trucking industry. Jones designed a portable air-cooling device, which included an undercarriage gasoline motor sturdy enough to handle the jolts of long-distance travel. Early modifications made the units even smaller and lighter, and moved them to the over-the-cab mount that is still in use on refrigeration trucks today. Suddenly, people in rural or isolated areas could have access to fresh produce, meat, and dairy items all year long. Further advancements soon led to standardized refrigerated containers which could be utilized on a truck, ship, or train, all without the need of unloading and repacking. The transport refrigeration industry boomed with the creation of these refrigerated boxcars, all of which used Jones technology. Together with Numero, who sold Cinema Supplies, Jones formed the U.S. Thermo Control Company, which grew rapidly in the 1940s. During World War II, the company provided refrigeration units that were used to help preserve not only food, but also blood and medicine for the military. In addition, U.S. Thermo Control cooling products were built into the cockpits of bombers and ambulance planes, and also provided air conditioning to personnel in field hospitals. Near the end of the war, Jones became the first African American inducted into the  American  Society  of Refrigeration Engineers, and by 1949, U.S. Thermo Control- which later became Thermo King- was worth several million dollars. Throughout the 1950s, Jones did consultant work for the Department of Defense, the Bureau of Standards, and other branches of the government. Although he is best known for his work with refrigeration units, during his lifetime, Frederick Jones patented over 60 inventions. He created X-ray machines, small and large engines, and sound equipment for radio and film production, generators, and even a machine that dispensed paper tickets. Jones passed away in Minneapolis, after a battle with lung cancer, on February 21, 1961. In 1977, he was inducted into the Minnesota Inventors Hall of Fame. Thirty years after his death, President George H.W. Bush awarded the National  Medal  of Technology posthumously to Jones and Numero, presenting the awards to their widows in the White House Rose Garden. Jones was the first African American to receive the National  Medal  of Technology. Sources â€Å"Frederick Jones.†Ã‚  Biography.com, AE Networks Television, 19 Jan. 2018, www.biography.com/people/frederick-jones-21329957.â€Å"Frederick McKinley Jones.†Ã‚  The Columbia Encyclopedia, 6th Ed, Encyclopedia.com, 2019, www.encyclopedia.com/people/science-and-technology/technology-biographies/frederick-mckinley-jones.â€Å"Frederick McKinley Jones.†Ã‚  Invent.org, National Inventors Hall of Fame, 2007, www.invent.org/hall_of_fame/343.html.â€Å"Frederick McKinley Jones: How Has He Transformed the Scene?†Ã‚  Richard G. (Gurley) Drew, www.msthalloffame.org/frederick_mckinley_jones.htm.

How to Write About Pets Writing a Book About Pets Step-by-Step

How to Write About Pets Writing a Book About Pets Step-by-Step How to Write About Pets: 6 Steps for Writing a Book About Pets the Right Way Writing about pets is a great way to share your passion and get paid for it!But thats only doable if you know how to write about pets in a way that others will actually want to readBecause lets be real, wed all love to gush about how amazing our pets are ALL day long, but thats not whats going to sell.I have some tips for writing a book about pets (or just writing in general) to help you out.Here are the steps for writing about pets:Journaling or free-writing about petsResearching writing about petsDevelop your pets characterDecide on the themeRead books about pets to learnBuild your pets author platformNOTE: If youre ready to start your book about pets, we can help you with that. In our VIP Self-Publishing Program, weve helped hundreds (even thousands) publish their books, even some about pets. Learn more about it hereHow to Write a Book About PetsIf you’re ever having a bad day at work, you may indulge in scrolling through some kind of social media app to get your mind off y our problems.As you scroll, something catches your eye, so you stop. It’s a video of cat with no front legs, learning how to jump, run, and play while still managing to be cute and adorable.You can’t help yourself; you smile.Not only is the kitty’s antics a little funny, but the story is also inspiring. Despite its disability, the cat forges on as if it had four legs instead of only two. Well, if that sweet little kitty can overcome its obstacle, you can get through your bad day at work.This is the power of pet stories.Along with making us laugh, pets and animals have a way of tugging at our heartstrings. Even though they’re animals, their tails- I mean, tales- humanize us every day.Pets and animals- big or small, hairy, feathered, covered with scales, paws, wings, or hooves- have a way of impacting our lives, whether it’s with humor or heroism.Either way, there’s a big market for pet stories and they give you a strong reason to write a book about them.Besides, anybody who has ever had pets always has a few stories to tell.So, do you think your pet/s have a unique story to share? Ive got some tips to help you share it.#1 Journaling or freewriting about your petsSet aside a few minutes each day- let’s say, 20 minutes or more- to write about your pets. Developing this writing habit is crucial to actually finish your project.Try to focus on one memorable event and write it down. This doesn’t need to be perfect; you can always revise later.If you are still feeling a bit stuck, try these ideas for writing about pets:Write about the time you met your pet for the first time. Were they given to you as a present? Did you adopt them from the shelter? Or did you find each other through some sort of happenstance?Write down something funny your pet did. Did they fail at training? Did they have an odd habit? Why was this memory significant to you? Was anyone else there with you and were they also amused or no?Write ab out a time you lost your pet. How did this affect you? How was their loss significant? What brought you two back together again? If your pet passed away, how did you handle your grief after?If you are still feeling stuck, try using these pet writing prompts to help you get some ideas to write down.#2 Research and notesJust like any other form of writing, you will need to backup your brainstorming with sound book research.This research will provide background information to your pet’s story to give it a fuller narrative and may help you to develop a theme (we’ll talk about themes next).Here are some research topics for pets and animals:Species/breeds: Research your pet’s species and breed. Does your pet fit these characteristics? Make notes of your pet’s behaviors and habits and see if they are common. How do they communicate (think sounds and body language)? Do other pet owners experience the same behaviors with their pets? This kind of research is espec ially important for exotic pets, like tarantulas, snakes, and turtles. It is unlikely that many readers of your story will have any kind of experience exotic species and/or breeds, so be sure to share more information with themService animals: If your pet was a service animal of some kind- therapy, police, military, leading the blind, search-and-rescue- research about those services provided and the organizations out there that provide them. These animals have benefited people tremendously and have very moving stories. If you have done any kind of professional and/or volunteer work with service animals, readers will find your insights and experiences invaluable.Adopted/rescue pets: Perhaps you adopted your pet from an animal shelter. Research the specific shelter you adopted your pet from, as well as how shelters functions in general. How high is the need to adopt animals? If your pet’s species or breed is one that has a high rate of ending up in shelters, it’s imperat ive to conduct research on this issue and provide readers information on it and how to prevent it. For example, pit bull terriers and huskies are two dog breeds that are known to often be sent to shelter; pit bull terriers are sent in because people use them for dog fighting and believed to be an aggressive breed, while huskies have extremely high energy and are very clever, both of which make them difficult to handle. This will encourage readers to think carefully about pets they adopt into their family and prepare for the responsibility they require. Perhaps you volunteered with a pet or animal sanctuary. Research the history and the purpose and mission of the organization.Pet care advice: Taking care of pets requires a great deal of responsibility. Each pet has its own set of care instructions, and some even require special care. What is the best way to care for this particular pet? What kind of expenses has your pet incurred? For example, let’s say you bottle-fed a kitten because it was an orphan. In your story, detail where you bought supplies for bottle-feeding, how often you fed them and how much for each feeding, how long you had to bottle-feed them, and at what age is best to finally transition from milk to solid food. Readers may find this information handy in the future.It may be wise to research and share some advice on how to encourage kids to be responsible for their pets.Sometimes kids are eager for a new pet, but once they realize how much work it is to take care of them, they quickly lose interest and neglect the pet they so badly wanted before.This is an issue that many parents face and often end up taking care of the pet themselves. It’s important to hold children accountable to their choices, but there are ways to do that without making them begin to dislike their pet.#3 Developing your pets characterIf your pet is still in your life, observe them and take notes. What are their habits? How do they interact with people and oth er animals? Do they do anything unique or peculiar? This research will enable you to develop your pet’s character and endear them to your reader.Don’t assume that just because you love your pet, your readers automatically will as well. This may be hard to believe, but it’s true. What makes your pet any different from others? You have to develop their character just as deeply and richly as you would a human character.Your pet’s story won’t stand out to readers unless their character stands out to them as well.Here’s some character development tips and advice to help you out:Detail their backgroundNote their strengths and weaknessesObserve unique habits or traitsCreate a character arc for themThe following excerpt from Marley by John Grogan is a great example of developing a pet’s character by using the rule of show, dont tell:â€Å"Just as we were reaching the car, we heard a commotion coming from the woods. Something was crashing thr ough the brush- and breathing heavily. It sounded like what you might hear in a slasher film. And it was coming our way. We froze, staring into the darkness. The sound grew louder and closer. Then in a flash the thing burst into the clearing and came charging in our direction, a yellow blur. A very big yellow blur. As it galloped past, not stopping, not even seeming to notice us, we could see it was a large Labrador retriever. But it was nothing like the sweet Lily we had just cuddled inside. This one was soaking wet and covered up to its belly in mud and burrs. Its tongue hung out wildly to one side, and froth flew off its jowls as it barreled past. In the split-second glimpse I got, I detected an odd, slightly crazed, yet somehow joyous gaze in its eyes. It was as though this animal had just seen a ghost- and couldn’t possibly be more tickled about it.â€Å"Then, with the roar of a stampeding herd of buffalo, it was gone, around the back of the house and out of sight. Jenn y let out a little gasp.â€Å"‘I think,’ I said, a slight queasiness rising in my gut, ‘we just met Dad.’†Even though we only see the daddy dog for a just brief moment- literally- we’ve learned something about John’s new puppy, Marley; he is going to be a big, wild, hard-to-handle, and happy dog.This scene is foreshadowing the kind of main character Marley will be later in the story.#4 Think of a themeNow that you have some done some substantial brainstorming and research, think of a theme your pet’s story could fall into. Themes in pet stories help connect ideas and issues with stories. Often our experiences with our pets coincide with life-changing events. If this is true for you, consider how your pet’s presence helped you through that time in your life.Examples of themes include coming-of-age, new relationships/romances, new parents, twenty-something years, thirty-something years, historical events, etc. You could eve n write a pet-themed cookbook with recipes for fun pet treats!#5 Read books about petsTo better understand the niche market of pet and animal stories, read books about pets.Here are some examples of books about pets you can learn from:Marley and Me: Life and Love with the World’s Worst Dog by John GroganSeabiscuit: An American Legend by Lauren HillenbrandDewey: The Small-Town Library Cat Who Touched the World by Vicki MyronAlex Me: How a Scientist and a Parrot Discovered a Hidden World of Animal Intelligence- and Formed a Deep Bond in the Process by Irene M. PepperbergFor more examples, you can check out thislist of animal memoirs on Goodreads.As you read, ask yourself these questions: What kind of impact did this animal have on the writer?What’s the theme of the story?What kind of research about this animal did the writer have to do?What does the writer do with this story that you like?What would you do differently in your pet’s story?#6 Build the pets onlin e platformYes, you did read that right. While many pets have an online platform, its necessary for yours to have one if youre writing about them.As you complete your pet’s story, begin building an online platform†¦for your pet. Having an established online platform will help market your story once you publish it, so come up with a plan on how to promote your story, and your pet.Here are some creative ways to create â€Å"buzz† about your upcoming book about your pet:Create an Instagram account for themBlog on your author website about themHave a bunch of videos of your pet? Make an online video seriesTheir online platform can be about anything- funny things they do, the two of you traveling together, throwing birthday parties for them, and so on. You can even write posts and captions from their point-of-view.In fact, this will even help you with building their character to make them more relatable to your audience.If you’re still feeling at a loss on how to do this, read some pet blogs and search social media for examples. They may give you an idea of what you need to do to get followers for your pet.Ready to write about your pet?Your pet’s story deserves to be heard. Start writing today. Give your pet a kiss on the head and put your fingers to the keyboard while you sign up for this training thatll help you make headway on your book today.

Non Essay Example | Topics and Well Written Essays - 1000 words

Non - Essay Example The painting has a symmetrical balance. The characters in the painting get provided with equal weight as the artist appears to have evenly divided the painting into two equal pieces by an imaginary line in the middle of the peace. The characters on each half of the painting also get drawn through the middle of each pane. The artist uses balance to evenly contrast the two emotions of the characters in the painting (Clarke 34). The artist uses a vertical color that divides the piece evenly into two. The artist uses one half to present the happy and smiling character, and the other half he employs to depict the sad character. The artist also employs the use of a curved white line around the hair of the character with a smiling face which assists to further depict the softening effect, pleasing, and sensual quality of the character. The artist uses a variety of color to communicate the emotions represented by the characters in the painting. One half of the painting gets dominated by brig ht colors while the other gets dominated by dark colors. On the character with a smiling face and what appears to be a happy mood, the artist employs the use of white tints of yellow, brown, blue, purple, and black. The use of a variety of tinted colors makes this half of the painting easily striking compared to the other half. ... The use of these shades provides the character with a heavy look. The artist employs the use of diagonal lines on both halves of the painting. On the background of each character, there appear diagonal lines with varying tint and shades. On the background of the happy character, the artist uses cross-cutting diagonal lines of white tint lines of clue. The background of the sad character gets characterized by cross-cutting diagonal lines of brown shade. The use of diagonal lines in the piece by the artist assists to create movement within the picture and thus create varying focal points within the artwork. The artist also draws a balance at the center of the piece by using an almost vertical line that consists of multiple colors of different shades and tints. Through the use of various colors and lines, the artist employs the use of texture throughout the painting. The half with a happy character has a smooth texture. The artist achieves this by using white tint of various colors. The white tint used on this half of the painting provides it with a light feel. The artist also seems to have employed the use of smooth and gentle brush strokes on this half of the painting (Clark 67). The artist also uses straight diagonal lines in the background of the character making this half of the painting to have an overall smooth feel. The use of dark shade on the half with the sad character provides the painting with a rough feel. The dark shade paint used provides a thick feel to this half of the painting. The shade on this half of the piece appears to have gotten created seem to have gotten created out of broad and impulse brush strokes. This provides the sad character with a dynamic and rough feel. Emphasis on the painting is on the contrast of the two characters within